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ea0059oc3.2 | Obesity & diabetes | SFEBES2018

Glucose regulates pancreatic β cell Ca²⁺ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

Salem Victoria , Suba Kinga , Martin-Alonso Aldara , Fernando Delgadillo Silva Luis , Akhtar Nadeem , Mousavy Neda , Georgiadou Eleni , Gaboriau David , Rothery Stephen , Stylianides Theodorus , Marchett Piero , Briant Linford , Ninov Nikolay , Hodson David , Distaso Walter , Rutter Guy

Background and Aims: β cell connectivity is a feature of pancreatic islets in vitro but its existence in vivo, when innervated and continuously perfused with blood, has not yet been demonstrated. We imaged islets engrafted in the anterior chamber of the mouse eye (ACE) to explore this question.Methods: Mouse (C57BL6, Ins1Cre::GCaM6mf/f) or human islets infected with adenovirus to express GCaMP6m, were engrafted and Ca<su...

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Endocrine Abstracts

Glucose regulates pancreatic β cell Ca²⁺ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

BiosciAbstracts

Endocrine Abstracts

Glucose regulates pancreatic β cell Ca2+ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

BiosciAbstracts

Glucose regulates pancreatic β cell Ca²⁺ dynamics and connectivity in vivo in the anterior chamber of the mouse eye